The Centre for Health Protection has confirmed a Kwong Wah Hospital doctor has tested negative for Middle East Respiratory Syndrome after returning from a medical exchange in Seoul at the end of May and falling ill.
Three other suspected cases have also tested negative for MERS.
Secretary for Food & Health Dr Ko Wing-man urged medical staff not to visit South Korea for exchange activities, adding that Government officials have cancelled planned healthcare meetings in Seoul this month.
Dr Ko also announced that with immediate effect, visitors to Hong Kong will be required to sign a health assessment form confirming that the information they provide is the truth, adding that they would be breaking the law by providing false information.
He said the new measure was being introduced after a Korean traveller who passed through Hong Kong last week tested positive for the MERS virus. He has since denied hiding his health details from officials at Hong Kong International Airport.
Dr Ko also urged travellers to take precautions when visiting MERS affected places and to avoid going to medical facilities unless absolutely necessary.